渐渐习惯了现代生活的美国队长史蒂夫·罗杰斯（克里斯·埃文斯 Chris Evans 饰），在一次行动后隐隐嗅到神盾局内部所弥漫出来的凶险气味。而当得知神盾局正秘密进行的“洞察计划”后，他更为此感到愤怒。某天，神盾局指挥官尼克-法瑞（塞缪尔·杰克逊 Samuel L. Jackson 饰）遭到一群武装分子袭击，他拼尽全力将一支U盘交到美国队长手中，这里面藏着该局二战以来最重要且最可怕的秘密。在此之后，美国队长因涉嫌杀害尼克而遭到前局长亚历山大·皮尔斯（罗伯特·雷德福/Robert Redford 饰）的通缉和追杀，逃亡中他得到黑寡妇（斯嘉丽·约翰逊 Scarlett Johansson 饰）和退伍老兵“猎鹰”（安东尼·麦凯 Anthony Mackie 饰）的帮助。
他们必须争分夺秒解开神盾局的秘密，阻止“洞察计划”的施行……
Lewis returns to Oxford after several years absence and is reluctantly assigned by his new boss, DCS Innocent, to the murder of an Oxford mathematics student who was shot while participating in a sleep study. The key-code used to access the sleep lab was assigned to a fellow maths student, Daniel Griffin, but Daniel's math tutor has provided him with an alibi. Daniel is a maladjusted young man who will soon inherit his father's automotive empire. He is disruptive and has no respect for his uncle who now heads the company. The future of the company however rests on an impending deal with Japanese investors who insist that family unity is all important at this time. When two other murder occurs, Lewis must decipher a cryptic clue left in an old case file by his former boss, Chief Inspector Morse.
